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Dfly620-SR
• Widefield and fluorescence
• Spinning disk confocal, and TIRF single molecule imaging microscope paired with a Leica DMi8 and High-Powered Laser Engine.
• The following excitation lines will be available: 405, 488, 560, 640, and 730.
• A Mosaic3 DMD for photostimulation experiments such as FRAP and photoactivation and conversion.
• MicroPoint Gen 4 for ablation, uncaging, FRAP, photo-conversion, activation and optogenetics.
• The following Leica objectives are available with the system: 20x air, 25x water immersion, 40x water immersion, 40x oil, 63x glycerol, and 100x TIRF.
• An Okolab stage-top incubation kit for environmental control of temperature, CO2, and humidity.
• Dual Camera imaging: ZL41 sCMOS and iXON EMCCD
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AXIO-OBZ1
- DIC, brightfield and fluorescence
- Halogen lamp 12V 100W for transmitted light and PhotoFluor LM-75 for reflected light
- Objective lenses: Plan-Neofluar 40X/1.3 NA oil DIC; Plan-Apochromat 100X/1.4 NA oil DIC
- Fluorescence filter sets: DAPI, FITC, Cy3, CFP and YFP
- Hamamatsu CMOS Orca Flash 4.0 LT camera
- MetaMorph acquisition and image analysis software
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DMi8
- SP8 laser Scanning confocal module
- Inverted, fully motorized
- Brightfield and fluorescence
- Incubation chamber and CO2 control.
- Halogen lamp 12V 100W for transmitted light and EL6000 Mercury lamp for epifluorescence
- Lasers: 405 nm, 488 nm, 561 nm and 639 nm
- Detectors: 2 PMT SP, 2 HyD SP
- Objective lenses: HC PL APO 10x/0.40 CS2, HC PL APO 20x/0.75 IMM CORR CS2 objective, HC PL APO 40x/1.10 W CORR CS2 0.65, and an HCX Plan Apochromat 63 3 /1.40-0.06 NA OIL
- Software: LAS X SP8 Control Software
- Training takes 1-2 hours depending on experience.
- Digital Light Sheet module—assisted usage only:
- Objectives: HC PL FLUOTAR 2.5x/0.07, HC PL FLUOTAR 5x/0.15, HC APO L10x/0.30 W DLS, HC FLUOTAR L25x/0.95 W DLS
- Mirrors: DLS TwinFlect 2.5mm, DLS TwinFlect 5mm
- Filters: Filter DLS 455-495, Filter DLS 504-545, Filter DLS 575-615, Filter DLS 405/488/552/638
- Sample holders: U-Shaped Capillaries, 20mm x 2.5mm, U-Shaped Capillaries, 20mm x 1.5mm, FEP Tube 10 x 25 cm
- Minimum session time is 3 hours.
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LSM710
- DIC, brightfield and fluorescence
- Halogen lamp 12V 100W for transmitted light and X-Cite 120 XL for epifluorescence
- Lasers: 405 nm diode laser, a multiline Argon (458/488/514 nm), and a HeNe (543 nm)
- Detectors: 1 T-PMT, 1 GaAsP detector, 2 highly sensitive low dark-noise PMT’s
- Objective lenses: Plan-Neofluar 10X/0.3 NA, Plan-Apochromat 20X/0.8 NA, Plan-Neofluar 40X/1.3 NA oil DIC, and a Plan-Apochromat 63X/1.4 NA oil DIC
- Zeiss Zen 3.1
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LSM980
- DIC, brightfield and fluorescence
- Halogen lamp 12V 100W for transmitted light and Colibri 5 Type RGB UV for epifluorescence
- Lasers: 405 nm, 488 nm, 561 nm and 639 nm
- Detectors: 1 T-PMT, 1 GaAsP detector, 2 MA-PMT’s, Airyscan 2 Multiplex with 4Y and 8Y
- Objective lenses: Plan-Neofluar 10X/0.3 NA, Plan-Neofluar 40X/1.2 NA Imm Corr DIC, and a C Plan-Apochromat 63X/1.4 NA oil DIC
- Zeiss Zen 3.3
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THUND-MO
The Leica Thunder microscope system which allows for computational clearing of thick samples such as model organisms. The Thunder system features high resolution black and white (Leica DFC9000 GT) mounted on an upright fluorescence Stereo Microscope (Leica DM6B). The system is also equipped with a micromanipulator/injection apparatus.
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THUND-T
The Leica Thunder microscope system which allows for computational clearing of thick and thin samples that are stained with fluorescent markers. The Thunder system features high resolution black and white (Leica DFC9000 GT) and color cameras (Leica DMC4500) mounted on an upright fluorescence microscope (Leica DM6B). The system also features an array of objectives including long working distance, dry, water immersion, oil immersion and glycerol immersion.
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